The aim of this study was to investigate the effects of acarbose addition to the ration of dairy cows on changes in ruminal fermentation characteristics and variations of acute phase proteins (APPs) in dairy cows with subacute ruminal acidosis (SARA). A total of 24 SARA positive Holstein cows that were divided into 4 groups containin 6 cows in each were used in the study. Treatments were 1) 1 mg of acarbose/kg of BW, 2) 2 mg of acarbose/kg of BW, 3) 3 mg of acarbose/kg of BW and 4) control, no additive. Rumen fluid samples were taken starting from immediately after morning feding and at 4-h intervals until 12 h period. Acarbose increased ruminal pH in all groups immediately after feeding compared to control group (P<0.05). Total volatile fatty acid (VFA) and molar percentages of propionate and butyrate increased (P<0.05), whereas percentage of acetate decreased (P<0.05) in acarbose administrated groups when compared with control group. Ruminal levels of lactate were extremely low and below the level of detection (<1 nM) after acarbose administration. Decreases in haptoglobin (Hp) and Serum Amyloid A (SAA) levels were found to be statistically significant (P<0.05) in acarbose administrated groups when compared with control group. Thus, it was concluded that acarbose may improve the ruminal function and accelerate the repair process of the organism.