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Fırat University Journal of Health Sciences (Veterinary)
2018, Cilt 32, Sayı 1, Sayfa(lar) 017-021
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Cryopreservation of Kangal Dog Semen in Commercial Extender (CaniPlus Freeze)
Alper KOÇYİĞİT
Cumhuriyet Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Sivas, TÜRKİYE
Keywords: Kangal dog, semen, cryopreservation, caniplus freeze

Various solutions are used as sperm extenders in order to keep dog spermatozoa for a short time or for freezing for a long time. In our study, it was aimed to determine the effect of commercial diluent on freezing-thawing spermatological parameters in freezing of Kangal dog sperm. For this purpose, a total of 32 ejaculates were taken from two Kangal dogs by massage method. After freezing, the semen was divided into two equal portions and the first portion was diluted with Tris solution and the second portion was diluted with CaniPlus Freeze diluent. spermatozoa were placed in the refrigerator (+4 ⁰C) and left for 1.5 hours. At the end of the equilibration period, the straws were frozen for 20 minutes at a height of 4 cm (-120 ⁰C) from the level of liquid nitrogen and stored therein by immersion into liquid nitrogen. After freezing, the semens were thawed at 38 ⁰C for 30 seconds and subjected to spermatological examination. In the study, the resulting motility ratios were 45% and 50%, respectively in the control and CaniPlus Freeze groups (P>0.05). Abnormal spermatozoon ratio was found to be 18.8% in control group and 16.4% in CaniPlus Freeze group (P>0.05). As a result, in addition to researchers, we are particularly considering that the use of commercial diluents by clinician veterinarians in dogs will be of practical benefit in semen storage studies.

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