In this study, determing the effects of antioxidants on the computer assisted sperm analysis (CASA), oxidative stress parameters and the DNA damage of the thawed sperm was aimed. Semen collected from 10 Pirlak rams was divided into five equal aliquots and diluted with the tris base extender containing taurine, trehalose, trolox, lipoic acid and no antioxidant (control), cooled to 5 ⁰C and frozen in 0,25 mL French straws in liquid nitrogen vapour and stored in liquid nitrogen. In the thawed semen, the highest values were detected in taurine group with regard to subjective motility and CASA motility, average path velocity μm/s (VAP) and straight linear velocity μm/s (VSL). The lowest values in spermatozoa with abnormal head, middle piece and acrosome were found in all antioxidant groups when compared with the control group. The increases in hypoosmotic eosin staining test (H+/E- type) spermatozoa were significant (P<0.05) in taurine, trehalose and lipoic acid groups compared with the control group. The lowest value in DNA damage was observed in trehalose and lipoic acid groups. In terms of malondialdehyde (MDA), lipoic acid and trolox, which had the lowest value, indicating that it provided preservation. In conclusion, the efficacies of taurine, trehalose, lipoic acid and trolox that were used as antioxidants for freezing ram semen show differences, and lipoic acid is more effective on reducing oxidative stress and DNA damage.