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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2022, Cilt 36, Sayı 3, Sayfa(lar) 218-223
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Investigation of Mycoplasma bovis in Bovine Lung Samples with Pneumonia by Real Time PCR
Sifa KARAHAN1, Ismail Hakki EKIN2
1Ministry of Agriculture and Forestry Adana Veterinary Control Institute, Bacteriology Laboratory, Adana, TÜRKİYE
2Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Microbiology, Van, TÜRKİYE
Keywords: Bovine, lung, Mycoplasma bovis, real time PCR

The aim of this study was to investigate Mycoplasma (M.) bovis in bovine lungs with pneumonia by real time polymerase chain reaction (PCR). In this study, 100 lung tissue samples taken from cattle with pneumonia that were macroscopically diagnosed and brought to Adana Veterinary Control Institute were used. Real time PCR was used for the detection of M. bovis in the samples and conventional bacteriological culture method was used for the detection of other bacterial agents. M. bovis was detected in 52 of 100 bovine lung samples by real time PCR. In 58 of these samples, various bacteriological agents were isolated and identified but bacterial agent was not isolated from 42 of lung samples by conventional bacteriological methods. Escherichia coli was detected in the highest rate (25%) after M. bovis while Pasteurella multocida, Klebsiella pneumoniae, Sphingomonas paucimobilis, Mannheimia haemolytica, Staphylococcus epidermidis and Staphylococcus spp. were isolated from 13%, 7%, 7%, 4%, 1% and 1% of the samples, respectively. The findings of the study indicated that M. bovis was an important causative agent of respiratory diseases in cattle, and that the presence of other bacterial agents should be considered in the diagnosis and treatment of the disease.

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