This study was conducted for increasing the freezing quality by suppressing the oxidative stress that occurs during the freezing of ram semen by suppressing the oxidative stress by Ginkgo Biloba, which is known for its high level of antioxidant property. For this purpose, semen collected from 6 Akkaraman breed rams was pooled and distributed equally to the control and experimental groups. After the experimental groups [Group 1: %4 GB, Group 2: %2 GB, Group 3: %1 GB, Group 4: %0.5 GB, Group 5: %0.25 GB ve Group 6: Control (%0 GB)] were formed, the diluted-cooled semen were subjected to the glycerolization-equilibration process, drawn into 0.25 ml mini straws and frozen in an automatic freezing device. Spermatological and kinematic analyzes were performed on frozen-thawed semen using CASA, and viability, acrosomal damage and mitochondrial membrane potential analyzes were performed on flow-cytometry. In addition, membrane integrity was determined with the HOS test, while vitamin, fatty acid and cholesterol levels were measured with oxidative stress analysis. As a result of the analyses, it was seen that the groups containing 0.25% and 0.50% GB provided an increase in total, progressive, rapid motility, vitality and high mitochondrial membrane potential compared to the control group. In conclusion; it has been determined that adding 0.25%-0.50% GB extract to semen extenders in rams increases the freezing quality of semen. Among the experimental groups, it was determined that the group containing 4% GB had a harmful effect on spermatozoa. As a result of oxidative stress analyses, except for 4% GB, all experimental groups increased GSH levels compared to the control. In addition, 1% GB GSH-Px, 0.25% and 1% GB CAT and 4% GB increased MDA levels compared to the control. Compared to the control group, the group containing 2% GB provided a significant decrease in MDA levels, and the group containing 4% GB provided a significant decrease in SOD levels. No difference were detected in vitamin, fatty acid and cholesterol levels between the experimental and control groups.