The aim of this study was to investigate Salmonella spp., that cause serious infections and food poisoning in humans and animals, in fish and water samples provided in various water sources in Elazığ and its vicinity. Intestinal contents of 145 fish (7 different species) caught in six different water sources were examined bacteriologically for the presence of Salmonella spp. DNA was extracted from five suspicious isolates grown in selective media and Polymerase Chain Reaction (PCR) amplification was carried out using specific primers derived from 16S rRNA gene of Salmonella. In the 1.5% agarose gel electrophoresis of the PCR products, all the isolates (3.45%) were confirmed as Salmonella spp. These results provide information on the pollution in regional water sources and therefore on the degree of contamination of fish that live in these sources. The isolation of Salmonella agents which cause various infections in humans suggests that the pollution of water sources can threaten human health directly. However, large-scale studies are needed in order to determine the potential of this threat.