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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi |
2014, Cilt 28, Sayı 1, Sayfa(lar) 019-024 |
[ Turkish ] [ Tam Metin ] [ PDF ] |
Determination of Nucleotide Partial Sequence of VP2 Region of Infectious Pancreatic Necrosis Virus Isolated from Ovarian Fluid of Rainbow Trout (Oncorhynchus mykiss, Walbaum 1792) in Turkey |
Metin GÜRÇAY1, Ayşe PARMAKSIZ1, Ahmet SAİT2, Selehattin GÜRÇAY3 |
1Veteriner Kontrol Enstitüsü, Elazığ, TÜRKİYE 2Veteriner Kontrol Enstitüsü, İstanbul, TÜRKİYE 3Su Ürünleri İstasyonu, Elazığ, TÜRKİYE |
Keywords: Infectious pancreatic necrosis virus, ovarian fluid, Rainbow trout, nucleotide sequence, viral protein VP2 region |
In this study, partial nucleotide sequence VP2 region of Infectious Pancreatic Necrosis Virus
(IPNV) viral protein was detected from isolated ovarian fluid of rainbow trout (Oncorhynchus mykiss
Walbaum,1792) and its genetic relation was investigated to the worldwide IPNV isolates. For this
purpose, IPNV was isolated from ovarial fluid from fish cages belon to private enterprises in Elaziğ
province and identified by RT-PCR method and subjected to partial nucleotide sequence for VP2
region viral protein for this isolate. The nucleotide and deduced amino acid sequence data reported
in this isolate have been deposited in GenBank with the following accession number: KC525214
ELAZIĞ. Phylogenetic analysis was made for this isolate. Phylogenetic analysis revealed that
KC525214 ELAZIĞ isolate was clustering within Genogroup 5 clade most closely related to the
Serotype A2 together with French, Norwegian, Danish, Taiwanese, Spanish and American isolates.
The findings indicated that the transfer of ovarian fluids is associated with the transmission and
spread of IPNV. The determination of partial nucleotide sequence VP2 region viral protein for IPNV
was performed with this study in Turkey. These results are of importance for further
epidemiological studies and IPNV outbreaks in Turkey.
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