The aim of this study was to investigate the effects of multi enzyme supplementation (protease, phytase, β-glucanase, xylanase) and sex ratio on performance and egg characterizations in quails fed on corn-based diets. In this study, a total of 100 Japonesse quails (Coturnix Coturnix Japonica) of 58 days of age were used according to 2x2 factorial experimental design. It was constituted that 4 groups to two different diet (enzyme supplemented (1 g/kg diet) and no enzyme supplemented) and two different sex (male/female) ratio (1/3 and 1/5). Multi enzyme was not supplemented to group 1 (Control 1/3) and quails were placed as one male and three female to each cage. Multi enzyme was not supplemented to group 2 (Control 1/5) and quails were placed as one male and five female to each cage. Multi enzyme was supplemented to group 3 (Enzyme 1/3) and quails were placed as one male and three female to each cage. Multi enzyme was supplemented to group 4 (Enzyme 1/5) and quails were placed as one male and five female to each cage. Multi enzyme supplementation improved quail / day production (P<0.001), feed conversion ratio (P<0.01), egg weight (P<0.01) and shell weight (P<0.001). Sex ratio (1/5) improved feed conversion ratio (P<0.01) and shell index (P<0.01). In conclusion, enzyme supplementation can improve performance and egg parameters in quails fed corn-based diet.