The aim of this study was to investigate expression of matrix (M) gene of RBOK vaccine strain of rinderpest virus (RPV) in eucaryotic cell. The M gene was cloned into TOPO XL cloning vector. The recombinant plasmid was cut with EcoRI and SalI enzymes to obtain the M gene. Then, the M gene was transferred into pCI-neo mammalian expression vector. So, the resulting recombinant plasmid was named as pCI-neo Matrix. Afterwards, the pCI-neo Matrix vector was transiently tranfected into Vero cells. The presence of the Matrix proteins was showed by Western Immunoblotting Assay demonstrating that Vero cells expressed a 38 kDa protein reacting with anti-M antibody.