In this study, we aimed to investigate the therapeutic effect of 4 different concentrations of bovine amniotic fluid (BAF) in a dry eye (DE) mouse model in terms of some clinical and histological parameters. The material of the study consisted of 60 BALB/c female mice that were randomly divided into 6 groups with equal numbers. In both eyes of the other 5 groups except the control were instilled 5 μL 0.2% BAC BAC (benzalkonium chloride),, twice a day for 14 days in order to form a DE model. Between 15th and 30th days, group 2 (DE group) received 5 μL 0.9% isotonic saline, group 3 20% BAF, group 4 35% BAF, group 5 50% BAF and group 6 100% BAF, three times a day. All groups were evaluated regarding blink rate and conjunctival goblet cell count during the study. Corneal epithelial thickness, corneal vascularization and inflammatory cells count were assessed by histopathological examination in all groups at the end of study. At the end of the study, the most effective results were obtained in 35%, 50% and 100% BAF for the blink rate; and in 35% BAF groups for conjunctival goblet cell count. It was determined that the corneal epithelial layers of the DE group subjects were significantly thinner and corneal vascularization and inflammatory cell count were increased. According to these parameters, the most effective results were obtained in 35% and 50% BAF groups. These results suggest that moderate and high concentrations of BAF have beneficial effects on clinical and cellular symptoms of DE.