Objective: In recent years, noninvasive tests have been developed by chronic hepatitis B patients, which may be an alternative to liver biopsy for predict histology. This study aimed to evaluate the relationship between the stage of fibrosis in liver biopsy and basic serum biochemical parameters of HbeAg negative chronic hepatitis B patients.

Materials and Methods: The patients who were diagnosed as chronic hepatitis B,are older than 18, HbeAg negative, with HBV DNA level >2000 IU/mL, underwent liver biopsy and under follow up at infectious diseases department of our hospital from August 01, 2012 to August 31, 2019 were included in this study. Demographic characteristics and laboratory results were collected from patient files retrospectively. Repetitive patients were excluded. The patients were divided into three groups as mild fibrosis (F≤2), moderate fibrosis (F3-4) and cirrhosis (F5-6) according to Ishak fibrosis score. The patients with fibrosis score 5-6 were excluded due to inadequate number of the patients.

Results: In this study, 242 patients were enrolled. Number of male were 154 (63.6%) and female were 88 (36.4%) with a mean age of 40.84±11.06. Fibrosis score was higher in male and older age. It is determined that, high serum alanine aminotransferase, aspartate aminotransferase, prothrombin time, gamma glutamyl transpeptidase, alpha-fetoprotein, HBV-DNA levels (P<0.001) and low platelet count is related to progression of score and stage (P<0.001).

Conclusion: These parameters can predict of significant fibrosis in chronic hepatitis B patients. Non-invasive tests reduce but not eliminate the necessity of the liver biopsy. They should be used together with the liver biopsy.