Objective: Prostate and ovarian cancers are among the most common types of cancer in Turkey. N- (p-amylcinnamoyl) anthranilic acid (ACA) is an inhibitor of both transient receptor potential melastatin-2 (TRPM2) channels and phospholipase A2 (PLA₂). Recent studies have reported that TRPM2 channels and PLA2 are associated with cancer. The aim of the study is to investigate anti-cancer mechanism of ACA in ovarian (A2780) and prostate (PC-3 and LNCaP) cancer cells.

Materials and Methods: A2780, PC-3 and LNCaP cell lines were used in the study. All cell lines were incubated with ACA at concentrations of 1 μM, 5 μM, 25 μM, 50 μM and 100 μM for 24 h. Changes in cell viability were examined by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) method. The inhibitory logarithmic concentration 50 (LogIC50) values were calculated according to MTT assay results.

Results: There was a significant decrease in survival of cancer cells (A2780, PC-3 and LNCaP) incubated with ACA for 24 hours (P<0.05). All concentrations of ACA added to the culture medium caused a decrease in A2780 cell viability (P<0.05), In PC-3 and LNCaP cells, all concentrations of ACA except 1 μM were found to reduce cell viability for 24 hours (P<0.05). Furthermore, when LogIC50 values were examined, it was determined that ACA showed the strongest cytotoxic activity on LNCaP cells at lower concentration.

Conclusion: ACA was found to have cytotoxic activity on the A2780, PC-3 and LNCaP cells. These findings suggest that ACA has antitumor properties.