Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi |
2023, Cilt 37, Sayı 2, Sayfa(lar) 146-150 |
[ Turkish ]
[ Tam Metin ]
[ PDF ]
|
Identification of Some Potentially Pathogenic Bacteria in Cloacal Swap Samples of Caged Birds Using Partial 16S rRNA Sequence Analysis |
Rahem KHOSHBAKHT |
Amol University of Special Modern Technologies, Faculty of Veterinary Medicine, Pathobiology, Amol, IRAN |
Keywords: PCR, Caged birds, 16SrRNA sequencing |
Caged birds can be reservoirs of various microorganisms in their gut microbiota some of which can be harmful to humans. Considering people's interests in keeping wild cage birds and the potential ability of wild cage birds to transmit bacteria to humans, the present study was conducted to investigate some bacterial agents in the microflora of caged birds by polymerase chain reaction (PCR) and sequence analysis. Altogether 68 samples from different birds were collected with sterile cotton swabs from different areas of Mazandaran province, Iran and evaluated for detection of bacteria by sequencing of partial 16SrRNA PCR product. In total, 11 samples were positive for PCR reaction using universal primers. PCR products of samples were sequenced and results were uploaded to NCBI with the accession numbers. Comparison of the sequences with other similar sequences showed the similarity of the sequences to Corynebacterium, Tessaracoccus and, Christensenella species. Results showed the possible role of caged birds in the circulation and transmission of some opportunistic pathogenic bacteria.
|
[ Turkish ]
[ Tam Metin ]
[ PDF ]
|