Guided bone regeneration (GBR) is a dental surgical prosedure used to stimulate bone growth in tissue defects adjacent to dental implants. This study purposed to evaluate the effect of dietary supplementation of Vitamin C (Vit C) on the osseointegration and periimplant bone regeneration after implant insertion surgery with GBR. Thirthy Sprague Dawley rats were enrolled in the study and they were divided into 3 groups as control, Vit C 1 and Vit C 2. Two different doses (5 mg/kg and 10 mg/kg) of Vit C were performed to the Vit C 1 and Vit C 2 groups, respectively. Ossoeintegration and periimplant bone tissue regeneration analysed by reverse torque method. No statistically significant difference was observed in the biomechanical reverse torque analysis in the implants of the groups. Although osseointegration was higher numerically in the Vit C 1 group, this was not statistically significant. Vit C supplementation did not increase the osseointegration with GBR (P>0.05) (P:0.920). If the numerical increase in our findings were significant, it may be considered to recommend Vit C supplementation to patients scheduled for implant surgery.