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Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi
2013, Cilt 27, Sayı 1, Sayfa(lar) 027-032
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Determination of Renal TRPM2 Channels Immunoreactivities in Enalapril Administrated Diabetic Rats
Fırat Üniversitesi, Tıp Fakültesi, Histoloji Embriyoloji Anabilim Dalı, Elazığ, TÜRKİYE
Keywords: Diabetic neuropathy, enalapril, TRPM2, immunohistochemistry

Objective: Diabetes is a lifetime metabolic disease which reduces quality of life and increases morbidity and mortality. In this study, it was aimed to investigate the effects of enalapril on renal damage in Streptozotocin (STZ) induced diabetic rat model. For this purpose, immune reactivity of melastatine-depended Transient Receptor Potential-2 (TRPM2) ion channels which have important effects on apoptotic process, were determined.

Material and Methods: A total of 21 adult- Wistar Albino male rats were divided into 3 groups. The rats in the first group were used as control. The second group of rats were injected a single dose of 60 mg/kg STZ intraperitoneally, dissolved in phosphate-citrate buffer (pH:4.5), whereas third group of rats were the enalapril administered group (5 mg/kg/day oral for 10 weeks) following diabetes. All rats were decapitated at the end of 10 weeks. Renal tissue were placed into the 10% formaldehyde and embedded in paraffin blocks. The avidin-biotin-peroxidase method was applied into the 5 μm paraffin section to evaluate TRPM2 channels immune reactivity. The intensity of TRPM2 channels immune reactivity was scored semiquantitatively as follows: low +1, moderate +2, strong +3.

Results: In light microscopic examination, TRPM2 immunoraectivity was found slightly increased (+1) in control group whereas, diabetic group showed widespread and intensive immune reactivity (+3). Treatment group was expressed moderate (+2) TRPM2 channels.

Conclusion: The results of this study showed enalapril administration provided a protective effect against diabetic renal damage by reducing TRPM2 channel immune reactivity, in which thought to has a role in apoptotic process and diabetic nephropathies.

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