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Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi
2023, Cilt 37, Sayı 1, Sayfa(lar) 066-079
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Sinapic Acid Ameliorates Cisplatin-Induced Peripheral Neuropathy: An In Vivo and In Vitro Study
Caner YILDIRIM1, Hatice YAMANER1, Mustafa ÖRKMEZ2, Sibel CANGİ3, Mehmet Akif BOZDAYI2, Demet TAŞDEMİR2, Sena CEVİK1
1Gaziantep University, Faculty of Medicine, Department of Physiology, Gaziantep, TÜRKİYE
2Gaziantep University, Faculty of Medicine, Department of Medical Biochemistry, Gaziantep, TÜRKİYE
3Gaziantep University, Faculty of Medicine, Department of Pathology, Gaziantep, TÜRKİYE
Keywords: Cisplatin, neuropathy, nociceptive behaviour experiments, sinapic acid, cytotoxicity, in vivo and in vitro study

Objective: Cisplatin is among the most effective antitumor agents. Nevertheless, the drug has severe side effects. Due to its cytotoxic properties, cisplatin has a restricted use. One of the most restricting factors in cancer chemotherapy is neurotoxicity. Because sinapic acid is an antioxidant and anti-inflammatory drug, the protective effects of sinapic acid on cisplatin neuropathy were investigated.

Materials and Methods: Spraque Dawley male rats were randomly divided into five groups: group I, normal control; group II, Sham; group III, CIS; group IV, CIS+SA 20 mg/kg; and group V, CIS + SA 40 mg/kg. After conclusion of the study, behavioral nociceptive studies and nerve conduction velocity measurements were conducted on animals. The animals were then sacrificed, and oxidative stress indicators and proinflammatory cytokine levels in the sciatic nerve tissue and blood were evaluated using the ELISA method. Histopathological staining of sciatic nerve tissue was performed. In addition, the possible effect of sinapic acid on the antitumor activity of cisplatin was evaluated by cytotoxicity experiments.

Results: Cisplatin-exposed rats showed somatosensory dysfunction, motor incoordination, and a reduction in sciatic nerve motoric conduction velocity. CMAP latency time increased. Biochemically, oxidative stress markers and proinflammatory cytokine levels were elevated. Histopathological evaluation revealed axon degeneration, edema, and fibrosis. Sinapic acid reduced oxidative stress and proinflammatory cytokine levels. Sinapic acid also restored CMAP and nerve fiber architecture.

Conclusion: Sinapic acid treatment ameliorated these adverse effects caused by cisplatin. In in vitro experiments, it was determined that sinapic acid was shown to have restorative properties without impairing the antitumoral efficacy of cisplatin.


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